phosphorylated acetyl coa carboxylase p acc  (Cell Signaling Technology Inc)

Journal: Journal of Functional Foods

Article Title: Alleviating effects of koji-fermented soybean extract on nonalcoholic steatohepatitis in mice through inactivation of acetyl-CoA carboxylase without inhibition of AMPK

doi: 10.1016/j.jff.2025.106927

Figure Lengend Snippet: Fig. 4. Effects of KFS on the hepatic AMPK–ACC axis in mice fed a MCD diet for 6 weeks. Hepatic AMPKα (A, B) and ACC (C, D) levels were assessed via immunoblot analysis of liver homogenates. (A, C) AMPKα, phosphorylated AMPKα (p-AMPKα), ACC and phosphorylated ACC (p-ACC) levels were assessed in liver homogenates prepared from the mice in each group. Gapdh was used as the loading control. (B, D) The graphs show the ratios of the phosphorylated forms of AMPKα and ACC. The data are presented as levels relative to those in the chow-fed group. Mean ± SD (n = 3). MCD, MCD diet group; MCD + KFS, MCD diet with KFS administration group. *** p < 0.001 and ** p < 0.01, and * p < 0.05 vs. MCD by Dunnett’s test.

Article Snippet: We used antibodies against AMP kinase α (AMPKα), phosphorylated AMPK (pAMPK), acetyl-CoA carboxylase (ACC) and phosphorylated ACC (p-ACC) (Cell Signaling Technology, Danvers, MA) (Table S3).

Techniques: Western Blot, Control

Journal: Animal Nutrition

Article Title: Different types of bile acids exhibit opposite regulatory effects on lipid metabolism in finishing pigs through bile acid receptors

doi: 10.1016/j.aninu.2024.12.003

Figure Lengend Snippet: Fig. 5. Effects of HDCA on the relative expressions of lipid metabolism-related factors in 3T3-L1 mature adipocytes. (A-B) Effects of HDCA on the levels of lipid metabolism-related gene expression; n ¼ 6. (C-D) Relative protein expressions and grayscale analysis of lipid metabolism-related factors in HDCA-treated groups; n ¼ 3. Values with different letters mean significant difference (P < 0.05). ACC ¼ acetyl-CoA-carboxylase; C/EBPa ¼ enhancer binding proteins alpha; FXR ¼ farnesoid X receptor; TGR5 ¼ Takeda G-protein-coupled receptor 5; SREBP-1c ¼ sterol regulatory element-binding protein-1c; FABP4 ¼ fatty acid binding protein 4; PPARG ¼ peroxisome proliferator-activated receptor gamma; HSL ¼ hormone-sensitive triglyceride lipase; p-HSL ¼ phosphorylated hormone-sensitive triglyceride lipase; p-ACC ¼ phosphorylated acetyl-CoA-carboxylase; HDCA ¼ hyodeoxycholic acid; GW4064 ¼ the farnesoid X receptor agonist.

Article Snippet: The primary antibodies against HSL (4107, Cell Signaling Technology, Danvers, MA, USA), p-HSL (45804, Cell Signaling Technology, Danvers, MA, USA), phosphorylated acetyl-CoAcarboxylase (p-ACC) (42801, Cell Signaling Technology, Danvers, MA, USA), Takeda G-protein-coupled receptor 5 (TGR5) (ab72608, Abcam, UK), peroxisome proliferator activated receptor gamma (PPARG) (2443, Cell Signaling Technology, Danvers, MA, USA), bactin (4967, Cell Signaling Technology, Danvers, MA, USA) and GAPDH (97166, Cell Signaling Technology, Danvers, MA, USA) were diluted to a concentration of 1:1000.

Techniques: Gene Expression, Binding Assay